sirna targeting 5 Search Results


sirna for c-myb targeting 5′-uaucaguucguccaggcag-3′  (Amaxa)
90
Amaxa sirna for c-myb targeting 5′-uaucaguucguccaggcag-3′
Sirna For C Myb Targeting 5′ Uaucaguucguccaggcag 3′, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna for c-myb targeting 5′-uaucaguucguccaggcag-3′/product/Amaxa
Average 90 stars, based on 1 article reviews
sirna for c-myb targeting 5′-uaucaguucguccaggcag-3′ - by Bioz Stars, 2026-04
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sirna targeting 5′-gcaatgactcagatgcata-3′  (Ribobio co)
90
Ribobio co sirna targeting 5′-gcaatgactcagatgcata-3′
<t>SPAG9</t> and ELK1 expression in HCC tissue specimens and cell lines. Notes: ( A ) IHC staining of SPAG9 and ELK1 in hepatocarcinomas and adjacent noncancerous liver tissues. Weak SPAG9 and ELK1 staining can be observed in the adjacent noncancerous liver tissues (left panel) compared to the strong SPAG9 cytoplasmic staining and ELK1 nuclear staining in cancerous tissues (right panel) (magnification: 200×). ( B ) Protein and ( C ) mRNA levels of SPAG9 and ELK1 were examined in HCCLM3, HuH7, and HepG2 cell lines using Western blot and qRT-PCR analyses, respectively. ( D ) Mann–Whitney U -testing of IHC scores (*** P <0.001) (the bars show standard error), and ( E ) Spearman rank correlation analysis of SPAG9 and ELK1 expression in HCC tissues. Abbreviations: SPAG9, sperm-associated antigen 9; IHC, immunohistochemistry; qRT-PCR, quantitative real-time polymerase chain reaction; ELK1, ETS-like gene 1, tyrosine kinase; HCC, hepatocellular carcinoma.
Sirna Targeting 5′ Gcaatgactcagatgcata 3′, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna targeting 5′-gcaatgactcagatgcata-3′/product/Ribobio co
Average 90 stars, based on 1 article reviews
sirna targeting 5′-gcaatgactcagatgcata-3′ - by Bioz Stars, 2026-04
90/100 stars
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sirna targeting 5"-gaagaugaagaaauuaaaaga-3  (Gene Design Inc)
90
Gene Design Inc sirna targeting 5"-gaagaugaagaaauuaaaaga-3
<t>SPAG9</t> and ELK1 expression in HCC tissue specimens and cell lines. Notes: ( A ) IHC staining of SPAG9 and ELK1 in hepatocarcinomas and adjacent noncancerous liver tissues. Weak SPAG9 and ELK1 staining can be observed in the adjacent noncancerous liver tissues (left panel) compared to the strong SPAG9 cytoplasmic staining and ELK1 nuclear staining in cancerous tissues (right panel) (magnification: 200×). ( B ) Protein and ( C ) mRNA levels of SPAG9 and ELK1 were examined in HCCLM3, HuH7, and HepG2 cell lines using Western blot and qRT-PCR analyses, respectively. ( D ) Mann–Whitney U -testing of IHC scores (*** P <0.001) (the bars show standard error), and ( E ) Spearman rank correlation analysis of SPAG9 and ELK1 expression in HCC tissues. Abbreviations: SPAG9, sperm-associated antigen 9; IHC, immunohistochemistry; qRT-PCR, quantitative real-time polymerase chain reaction; ELK1, ETS-like gene 1, tyrosine kinase; HCC, hepatocellular carcinoma.
Sirna Targeting 5" Gaagaugaagaaauuaaaaga 3, supplied by Gene Design Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna targeting 5"-gaagaugaagaaauuaaaaga-3/product/Gene Design Inc
Average 90 stars, based on 1 article reviews
sirna targeting 5"-gaagaugaagaaauuaaaaga-3 - by Bioz Stars, 2026-04
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sirna targeting the 5-ht 2a r  (Shanghai GenePharma)
90
Shanghai GenePharma sirna targeting the 5-ht 2a r
Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly <t>through</t> <t>5-hydroxytrypta-mine-2A</t> receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control <t>siRNA</t> or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.
Sirna Targeting The 5 Ht 2a R, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna targeting the 5-ht 2a r/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
sirna targeting the 5-ht 2a r - by Bioz Stars, 2026-04
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sirna targeting 5′-ggccgagtgtactacttcadtdt  (Qiagen)
90
Qiagen sirna targeting 5′-ggccgagtgtactacttcadtdt
Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly <t>through</t> <t>5-hydroxytrypta-mine-2A</t> receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control <t>siRNA</t> or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.
Sirna Targeting 5′ Ggccgagtgtactacttcadtdt, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna targeting 5′-ggccgagtgtactacttcadtdt/product/Qiagen
Average 90 stars, based on 1 article reviews
sirna targeting 5′-ggccgagtgtactacttcadtdt - by Bioz Stars, 2026-04
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sirnas targeting 5-htr1a  (Ribobio co)
90
Ribobio co sirnas targeting 5-htr1a
Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly <t>through</t> <t>5-hydroxytrypta-mine-2A</t> receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control <t>siRNA</t> or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.
Sirnas Targeting 5 Htr1a, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirnas targeting 5-htr1a/product/Ribobio co
Average 90 stars, based on 1 article reviews
sirnas targeting 5-htr1a - by Bioz Stars, 2026-04
90/100 stars
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non-targeting (5′-uucuccgaacgugucacgutt-3′) sirnas  (Shanghai GenePharma)
90
Shanghai GenePharma non-targeting (5′-uucuccgaacgugucacgutt-3′) sirnas
Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly <t>through</t> <t>5-hydroxytrypta-mine-2A</t> receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control <t>siRNA</t> or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.
Non Targeting (5′ Uucuccgaacgugucacgutt 3′) Sirnas, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/non-targeting (5′-uucuccgaacgugucacgutt-3′) sirnas/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
non-targeting (5′-uucuccgaacgugucacgutt-3′) sirnas - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


SPAG9 and ELK1 expression in HCC tissue specimens and cell lines. Notes: ( A ) IHC staining of SPAG9 and ELK1 in hepatocarcinomas and adjacent noncancerous liver tissues. Weak SPAG9 and ELK1 staining can be observed in the adjacent noncancerous liver tissues (left panel) compared to the strong SPAG9 cytoplasmic staining and ELK1 nuclear staining in cancerous tissues (right panel) (magnification: 200×). ( B ) Protein and ( C ) mRNA levels of SPAG9 and ELK1 were examined in HCCLM3, HuH7, and HepG2 cell lines using Western blot and qRT-PCR analyses, respectively. ( D ) Mann–Whitney U -testing of IHC scores (*** P <0.001) (the bars show standard error), and ( E ) Spearman rank correlation analysis of SPAG9 and ELK1 expression in HCC tissues. Abbreviations: SPAG9, sperm-associated antigen 9; IHC, immunohistochemistry; qRT-PCR, quantitative real-time polymerase chain reaction; ELK1, ETS-like gene 1, tyrosine kinase; HCC, hepatocellular carcinoma.

Journal: OncoTargets and therapy

Article Title: SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression

doi: 10.2147/OTT.S98727

Figure Lengend Snippet: SPAG9 and ELK1 expression in HCC tissue specimens and cell lines. Notes: ( A ) IHC staining of SPAG9 and ELK1 in hepatocarcinomas and adjacent noncancerous liver tissues. Weak SPAG9 and ELK1 staining can be observed in the adjacent noncancerous liver tissues (left panel) compared to the strong SPAG9 cytoplasmic staining and ELK1 nuclear staining in cancerous tissues (right panel) (magnification: 200×). ( B ) Protein and ( C ) mRNA levels of SPAG9 and ELK1 were examined in HCCLM3, HuH7, and HepG2 cell lines using Western blot and qRT-PCR analyses, respectively. ( D ) Mann–Whitney U -testing of IHC scores (*** P <0.001) (the bars show standard error), and ( E ) Spearman rank correlation analysis of SPAG9 and ELK1 expression in HCC tissues. Abbreviations: SPAG9, sperm-associated antigen 9; IHC, immunohistochemistry; qRT-PCR, quantitative real-time polymerase chain reaction; ELK1, ETS-like gene 1, tyrosine kinase; HCC, hepatocellular carcinoma.

Article Snippet: SPAG9 -specific siRNA targeting 5′-GCAATGACTCAGATGCATA-3′, ELK1 -specific siRNA targeting 5′-CACATCCCTTCTATCAGCGTGGATG-3′, and control scrambled siRNA were chemically synthesized from RIBO-BIO (1451493202, S151113152330; Guangzhou, People’s Republic of China).

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, Quantitative RT-PCR, MANN-WHITNEY, Real-time Polymerase Chain Reaction

The relationship between SPAG9 expression and the clinical features of HCC

Journal: OncoTargets and therapy

Article Title: SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression

doi: 10.2147/OTT.S98727

Figure Lengend Snippet: The relationship between SPAG9 expression and the clinical features of HCC

Article Snippet: SPAG9 -specific siRNA targeting 5′-GCAATGACTCAGATGCATA-3′, ELK1 -specific siRNA targeting 5′-CACATCCCTTCTATCAGCGTGGATG-3′, and control scrambled siRNA were chemically synthesized from RIBO-BIO (1451493202, S151113152330; Guangzhou, People’s Republic of China).

Techniques: Expressing

Reduction in SPAG9 expression suppresses cell migration and invasion of HCC cell lines. Notes: ( A and B ) SPAG9 siRNA-treated cells had decreased mRNA ( A ) and protein ( B ) levels compared to the control cells. ( C ) Results from the wound-healing assay showed that SPAG9 depletion decreased cell migration of HCCLM3 and HuH7 cells. ( D ) Results from the transwell migration and matrigel invasion assay showed that SPAG9 depletion decreased cell migration and invasion of HCCLM3 and HuH7 cells; * P <0.05, ** P <0.01. Abbreviations: SPAG9, sperm-associated antigen 9; siRNA, small interfering RNA; HCC, hepatocellular carcinoma; h, hour.

Journal: OncoTargets and therapy

Article Title: SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression

doi: 10.2147/OTT.S98727

Figure Lengend Snippet: Reduction in SPAG9 expression suppresses cell migration and invasion of HCC cell lines. Notes: ( A and B ) SPAG9 siRNA-treated cells had decreased mRNA ( A ) and protein ( B ) levels compared to the control cells. ( C ) Results from the wound-healing assay showed that SPAG9 depletion decreased cell migration of HCCLM3 and HuH7 cells. ( D ) Results from the transwell migration and matrigel invasion assay showed that SPAG9 depletion decreased cell migration and invasion of HCCLM3 and HuH7 cells; * P <0.05, ** P <0.01. Abbreviations: SPAG9, sperm-associated antigen 9; siRNA, small interfering RNA; HCC, hepatocellular carcinoma; h, hour.

Article Snippet: SPAG9 -specific siRNA targeting 5′-GCAATGACTCAGATGCATA-3′, ELK1 -specific siRNA targeting 5′-CACATCCCTTCTATCAGCGTGGATG-3′, and control scrambled siRNA were chemically synthesized from RIBO-BIO (1451493202, S151113152330; Guangzhou, People’s Republic of China).

Techniques: Expressing, Migration, Control, Wound Healing Assay, Invasion Assay, Small Interfering RNA

SPAG9 regulates ELK1 expression and p38 activation in HCC cell lines. Notes: ( A ) Western blot and ( B ) qRT-PCR analyses revealed that siRNA knockdown of SPAG9 decreased protein and mRNA levels of SPAG9 and ELK1 , respectively, in HCCLM3 and HuH7 cell lines. ( C ) Western blot analysis showed that SPAG9 depletion decreased SPAG9 and p-p38 levels in HCCLM3 and HuH7 cells. Abbreviations: SPAG9, sperm-associated antigen 9; siRNA, small interfering RNA; qRT-PCR, quantitative real-time polymerase chain reaction; ELK1, ETS-like gene 1, tyrosine kinase; p-p38, phosphorylated protein-38; HCC, hepatocellular carcinoma.

Journal: OncoTargets and therapy

Article Title: SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression

doi: 10.2147/OTT.S98727

Figure Lengend Snippet: SPAG9 regulates ELK1 expression and p38 activation in HCC cell lines. Notes: ( A ) Western blot and ( B ) qRT-PCR analyses revealed that siRNA knockdown of SPAG9 decreased protein and mRNA levels of SPAG9 and ELK1 , respectively, in HCCLM3 and HuH7 cell lines. ( C ) Western blot analysis showed that SPAG9 depletion decreased SPAG9 and p-p38 levels in HCCLM3 and HuH7 cells. Abbreviations: SPAG9, sperm-associated antigen 9; siRNA, small interfering RNA; qRT-PCR, quantitative real-time polymerase chain reaction; ELK1, ETS-like gene 1, tyrosine kinase; p-p38, phosphorylated protein-38; HCC, hepatocellular carcinoma.

Article Snippet: SPAG9 -specific siRNA targeting 5′-GCAATGACTCAGATGCATA-3′, ELK1 -specific siRNA targeting 5′-CACATCCCTTCTATCAGCGTGGATG-3′, and control scrambled siRNA were chemically synthesized from RIBO-BIO (1451493202, S151113152330; Guangzhou, People’s Republic of China).

Techniques: Expressing, Activation Assay, Western Blot, Quantitative RT-PCR, Knockdown, Small Interfering RNA, Real-time Polymerase Chain Reaction

SPAG9-induced migration and invasion of HCC cells is dependent on ELK1. Notes: ( A ) Transwell migration and matrigel invasion assay revealed that ELK1 transfection rescued the inhibition in cell migration and invasion induced by SPAG9 depletion. ( B ) The expression of ELK1 was significantly upregulated in the siCtrl + ELK group and siSPAG9+ ELK1 group compared to the controls. ( C ) Transwell migration and matrigel invasion assay revealed that ELK1 depletion decreased the migration and invasion in HuH7 cells. ( D ) Western blot showed that siRNA knockdown of ELK1 decreased the protein level of ELK1, while SPAG9 expression was not changed in HuH7 cells. * P <0.05. Abbreviations: SPAG9, sperm-associated antigen 9; siSPAG9, SPAG9 siRNA; siCtrl, control siRNA; siRNA, small interfering RNA; ELK1, ETS-like gene 1, tyrosine kinase; HCC, hepatocellular carcinoma.

Journal: OncoTargets and therapy

Article Title: SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression

doi: 10.2147/OTT.S98727

Figure Lengend Snippet: SPAG9-induced migration and invasion of HCC cells is dependent on ELK1. Notes: ( A ) Transwell migration and matrigel invasion assay revealed that ELK1 transfection rescued the inhibition in cell migration and invasion induced by SPAG9 depletion. ( B ) The expression of ELK1 was significantly upregulated in the siCtrl + ELK group and siSPAG9+ ELK1 group compared to the controls. ( C ) Transwell migration and matrigel invasion assay revealed that ELK1 depletion decreased the migration and invasion in HuH7 cells. ( D ) Western blot showed that siRNA knockdown of ELK1 decreased the protein level of ELK1, while SPAG9 expression was not changed in HuH7 cells. * P <0.05. Abbreviations: SPAG9, sperm-associated antigen 9; siSPAG9, SPAG9 siRNA; siCtrl, control siRNA; siRNA, small interfering RNA; ELK1, ETS-like gene 1, tyrosine kinase; HCC, hepatocellular carcinoma.

Article Snippet: SPAG9 -specific siRNA targeting 5′-GCAATGACTCAGATGCATA-3′, ELK1 -specific siRNA targeting 5′-CACATCCCTTCTATCAGCGTGGATG-3′, and control scrambled siRNA were chemically synthesized from RIBO-BIO (1451493202, S151113152330; Guangzhou, People’s Republic of China).

Techniques: Migration, Invasion Assay, Transfection, Inhibition, Expressing, Western Blot, Knockdown, Control, Small Interfering RNA

Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly through 5-hydroxytrypta-mine-2A receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control siRNA or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.

Journal: International Journal of Molecular Medicine

Article Title: Effects of ketanserin on experimental colitis in mice and macrophage function

doi: 10.3892/ijmm.2016.2486

Figure Lengend Snippet: Ketanserin inhibits the expression of pro-inflammatory cytokines and influences the polarization of macrophages partly through 5-hydroxytrypta-mine-2A receptor (5-HT 2A R)/nuclear factor-κB (NF-κB). Bone marrow-derived macrophages (BMDMs) were transfected with control siRNA or 5-HT 2A R siRNA followed by stimulation with LPS plus 5-HT (LPS/5-HT). Relative mRNA level expression of (A) interleukin (IL)-1β, (B) necrosis factor-α (TNF-α), (C) IL-6 and (D) iNOS was evaluated in BMDMs by RT-qPCR. (E) Changes in pNF-κB p65 levels in LPS/5-HT-treated BMDMs were evaluated by western blot analyses. (F) Protein levels of pNF-κB p65 were quantified by densitometric analysis. The graphs depict the means ± SD of 3 independent experiments. * p<0.05 and ** p<0.01 vs. LPS/5HT with ket-treated BMDMs.

Article Snippet: siRNA targeting the 5-HT 2A R (Genepharm Biotech, Shanghai, China) was synthesized (GACAACUGUCGUGAUUAUUTT) and control siRNA (UUCUCCGAACGUGUCACGUTT) was also used.

Techniques: Expressing, Derivative Assay, Transfection, Control, Quantitative RT-PCR, Western Blot